The proliferation and differentiation of porcine adipose-derived stem cells by keratins incorporated with chitosan-Az
Date Issued
2014
Date
2014
Author(s)
Huang, Yao-Chang
Abstract
Advances in the extraction, purification, and understanding of the characterization of keratin proteins from hair and wool let researchers continuously study the application of keratin-based biomaterials. Keratins have intrinsic bioactivity, biodegradability, and biocompatibility materials. Keratins are also convenient, natural biomaterials due to the source of keratins is convenient and cheap. In the other hand, issue of bone repair clinically is very popular, especially regeneration of large traumatic bone defects. Therefore, using of regenerative biomaterials has been extensively studied for bone tissue engineering.
In this study, we hope to use keratins as biomaterials base, and mix with chitosan which is modified to enhance the mechanical property of keratins. Then we cultured cell to the keratin-chitosan substrates, and observe the ability of cell adhesion and proliferation. Furthermore, we added differentiation-inducing factors to induce stem cells to become osteoblasts and observe it, and we wish to support some contribution to bone tissue engineering.
First, we extracted keratins from human hair, and used SDS-PAGE to confirm the protein whether the keratins which we wanted to get. Then we mixed with chitosan which is crosslinked by photochemical to strengthen the mechanical property of keratins, and fabricated the films and scaffolds with keratin-chitosan composite materials. After seeding cell on the films or scaffolds, we observe the situation of cell adhesion and proliferation on the films and scaffolds, respectively. We further induced and observed the stem cells to differentiate osteoblasts. Through our research, we succeeded to obtain the keratins from human hair, and these keratins could enable to provide suitable environment for cell attachment and proliferation. We also improved the keratin weak mechanical properties after mixing with chitosan-az. In the differentiation aspect, we could find the morphology of cells that is different with original cells, and we showed the stem cells to convert to osteoblasts, and still keeping growing up.
Our study successfully demonstrates keratins that have wonderful bioactive and biocompatibility materials. Keratins promote cell attachment, proliferation and differentiation. Hence, keratins may still have huge potential of development in tissue engineering in the future.
Subjects
角蛋白
幾丁聚醣
膜
支架
硬骨組織工程
脂肪幹細胞
Type
thesis
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