Functional study of Drought-Induced protein 19-2 in Arabidopsis thaliana
Date Issued
2011
Date
2011
Author(s)
Chun, Chun-Wei
Abstract
Plants as sessile organisms must respond to changes in environmental conditions, such as various environmental stresses including too little water (drought), too much salt (salinity), and extreme temperature. The family of drought-inducible 19 (Di19) contain seven member with unknown functions in Arabidopsis. The AtDi19 family contains two atypical Cys/His zinc finger-like domains which may participate in different stress response and signaling pathways. AtDi19-2 (At1G02750) transcript level increased in response to high-salt stress, but its protein expression pattern and functions remain unknown. Previous study suggested that AtDi19-2 can interact with 14-3-3 proteins and NUC/AtIDD8 by use of tandem affinity purification and pull-down assay. Here, I use Bimolecular fluorescence complementation (BiFC) assays and quartz crystal microbalance (QCM), and confirmed that AtDi19-2 can interact with 14-3-3 proteins in nucleus and cytosol, and interact with NUC in the nucleus. In addition, I found that di19-2 di19-5 double mutant have higher germination rate than wild-type under condition of salt stress. This suggested that AtDi19-2 or AtDi19-5 may play important role in salt stress response.
Subjects
Drought-inducible 19
NUTCRACKER
bimolecular fluorescence complementation
quartz crystal microbalance
salt stress
Type
thesis
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