Genetic and Functional Study of the Rat Seminal Vesicle Secretion Protein III, RSVS III
Date Issued
2004
Date
2004
Author(s)
Lee, Chin-Mei
DOI
zh-TW
Abstract
Rat seminal vesicle secretion protein contains five well-defined major components designated RSVS I-V in decreasing order of molecular weight.
RSVS I-III were covalent cross-linked to form high molecular complexes by transglutaminase. RSVS III was confirmed to be derived from RpSV-1 mRNA. RSVS III was immunolocalized in the luminal epithelium of seminal vesicle and the copulatory plug.
The genomic structure of RpSv-1(RSVS III?was analyzed to establish a 5’-flanking region of 1771 bp, three exons of 92, 1482 and 327 bp, and two introns of 230 and 89 bp. The transcription unit is organized with the first exon encoding a signal peptide, and the second exon encoding a protein in its entirety, whereas the third encompasses a 3’-untranslated region, that shares common features with the arrangement of transcription units of the rapid evolving substrate of tansglutaminase (REST) gene family.
The RSVS III?gene was mapped to chromosome 3q42 locus. We found another paralog, RSVS IIIβ gene, which shares 99.9% nucleotide sequence identity to RSVS III?in the same locus. Northern blotting and RT-PCR were performed to demonstrate the exclusive expression of this duplicated pair in seminal vesicle with a ratio of RSVS III?mRNA to RSVS IIIβ mRNA ≒ 2.0. The mRNA expression of RSVS III? RSVS IIIβ were stimulated by testosterone. The genes in human chromosome 20q13, mice chromosome 2H3 and rat chromosome 3q42 were identified to have the arrangement of their transcription units similar to a REST gene. The molecular evolution of these REST genes is discussed.
Subjects
基因演化
轉麩胺醯胺酸酶
儲精囊
交配栓
copulatory plug
transglutaminase
seminal vesicle
REST gene evolution
Type
other
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