Enhancing Immunity by Dietary Consumption of a Probiotic Lactic Acid Bacterium (Bifidobacterium Lactis Hn019): Optimization and Definition of Cellular Immune Responses
Resource
EUROPEAN JOURNAL OF CLINICAL NUTRITION v.54 n.11 pp.849-855
Journal
EUROPEAN JOURNAL OF CLINICAL NUTRITION
Journal Volume
v.54
Journal Issue
n.11
Pages
849-855
Date Issued
2000
Date
2000
Author(s)
Chiang, B L
Sheih, Y H
Wang, L H
Liao, C K
Gill, H S
Abstract
Objective: To define the cellular basis for immune enhancement by a probiotic lactic acid bacteria strain ( Bifidobacterium lactis HN019); and to determine whether immune enhancement can be optimized by delivery in oligosaccharide-enriched low-fat milk. Design: A double- blind, three- stage before-and-after intervention trial. Setting: Taipei Medical College Hospital, Taipei, Taiwan. Subjects: Fifty healthy Taiwanese citizens (age range 41-81; median 60) randomly allocated to two groups. Interventions: In stage 1 (run-in control stage) all subjects consumed reconstituted low- fat milk (LFM) for 3 weeks; in stage 2 ( probiotic intervention) subjects consumed B. lactis in LFM ( group A) or B. lactis in lactose-hydrolysed LFM (group B) for 3 weeks; in stage 3 all subjects returned to non- supplemented LFM for a further 3 weeks (washout stage). The innate immune functions of two different leucocyte types ( polymorphonuclear (PMN) cells and natural killer (NK) cells) were assessed at four time points via in vitro analyses on peripheral blood samples; Results: While consumption of LFM alone had no significant effect on immune responses, stage 2 results indicated significantly enhanced PMN cell phagocytosis and NK cell tumour killing activity following consumption of milk containing B. lactis. These increases levelled off following cessation of B. lactis consumption, but remained above the pre-treatment values. Increases in PMN and NK cell activity were greatest among subjects who consumed B. lactis in lactose- hydrolysed LFM. Conclusions: Dietary consumption of the probiotic bacterium B. lactis HN 019 enhanced immune function of two different types of leucocytes; the degree of enhancement was increased by consuming B. lactis in an oligosaccharide-rich substrate.
Subjects
Bifidobacterium lactis
LAB
probiotic
PMN cells
phagocytosis
NK cells
Type
journal article