Family-based Association Study of Hepatocellular Carcinoma Susceptibility Gene on Chromosome 4q: Preliminary Results
Date Issued
2006
Date
2006
Author(s)
Wu, Tai-Wei
DOI
en-US
Abstract
Abstract
Background High fre uencies of allelic loss in hepatocellular carcinomas (HCCs) were usually observed on chromosome 4q. Previous linkage analysis had found a linkage signal on chromosome 4q25. The aim of this study was to perform a family-based association study on chromosome 4q25 for further narrowing down the region that may contain HCC-susceptibility locus.
Method The sample consisted of 163 singleton families and 35 multiplex families from northern Taiwan. A total of 25 single nucleotide polymorphism (SNP) markers with average intermarker distance of ~250 kb were genotyped. Both pedigree disequilibrium test (PDT) and transmission disequilibrium test (TDT) were applied. We also investigated the association between SNPs and age at onset of HCC through Quantitative PDT.
Results The most significant signal was detected at SNP rs1514728 by the PDT (P=0.0038). Haplotype analyses revealed a haplotype T-T at SNPs rs1514728 and rs2271590 that was associated with HCC (empirical P =0.015). In stratified analysis, rs1514728 showed a significant association with early-onset HCC defined as diagnosis of HCC at younger than 43 years of age (P =0.024), while rs2704108 was associated with late-onset HCC (P =0.037). Results from TDT were generally compatible with the results from PDT. Quantitative PDT analysis also revealed a significant association between rs2704108 and age at onset of HCC (P =0.032).
Conclusion Consistent with previous linkage and association study, our findings suggested that the linkage region at chromosome 4q25 might contain one or more susceptible loci of HCC.
Subjects
肝細胞癌
相關性研究
hepatocellular carcinoma
association study
PDT
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-95-R93842007-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):edfb58ae31eaaebb092d684d2f81dfe9