Electric Kinetic Behavior of a Charge-regulated Particle in Gel Electrophoresis
Date Issued
2014
Date
2014
Author(s)
Huang, Chih-Hua
Abstract
Gel electrophoresis is adopted gradually and widely in all kinds of biochemistry experiments in recent decades and even more than conventional electrophoresis because the hydrodynamic effect arising from the liquid drag and the steric effect from particle-polymer interaction reduce the mobility of sample particles to make the observation easier and enhance the degree of accuracy. Besides in this study, we apply the finite element method to simulate gel electrophoresis and try to investigate the difference between simulations and real experiments, e.g., the changing of dielectric constant caused by the addition of gel. Then we still consider the particle property as charge-regulated for the similarity of real experiments and analyze the results of simulation to understand the difference and the advantage of gel electrophoresis, for instance, high gel weight percentage causes the weakness of the electric double layer polarization and various pH value or electrolyte (buffer solution) concentration affects the variation of the electric kinetic behavior. Eventually we match up the trend of real experiments and consider the Joule effect caused by applied electric field and the temperature effect to design different environmental temperature. Then the analysis of electric kinetic behavior of a charge-regulated under various temperatures also includes in this study. The whole thesis mainly indicates that the dielectric constant of the solution decreases by increasing the gel concentration then also affects if electric double layer polarization appears. Furthermore we also analyze the three various stages of electric kinetic behavior of a charge-regulated particle and eventually investigate how the temperature effect to affect a charge-regulated particle to emerge all various kinds of electric kinetic behaviors.
Subjects
凝膠電泳
介電係數
表面可解離
電雙層極化效應
焦耳效應
溫度效應
Type
thesis
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