The study of microfluidic shear stress on endothelial cells and amniotic fluid stem cells differentiation
Date Issued
2008
Date
2008
Author(s)
Chiang, Ya-Yu
Abstract
Research of stem cells for cardiovascular diseases is of growing interest. The miniaturized and portable micro bio-devices fabricated by micromachining technology is able to provide in-depth understanding of biological mechanism and activities related to cardiovascular diseases. The objective of this research is to study the influence oficrofluidic shear stress to the culture of ECs, and AFSC differentiation to ECs. Design and fabrication of micro devices are key to success for the experiment, as well as the integration of real-time monitoring optical and culturing systems. The experiment of cell culture was carried out over 24 hours. It was found that, under the microfluidic stimulation of shear stress, endothelial cells were successfully adhered on the substrate, stretched and aligned along with constant flows applied. The microfluidic shear stresses were made at 3, 6, 12, 18 dynes/cm2, respectively.his work shows the result of cell morphology and cell arrangement under the fluidic shear stress. 80% of endothelial cells were mostly found in morphology and arrangement aligned along the flow direction. Meanwhile, stretched cells were found in less than 20° out of the flow direction at the fluidic shear stress of 12 ynes/cm2. In addition, the migration speed of endothelial cells was also measured to be about 0.4 um/min. at the shear stress of 3 and 6 dynes/cm2, and 0.8 um/min. at the shear stress of 12 and 18 dynes/cm2, respectively.iological and fluidic stimulations of AFSC ifferentiation to endothelial cells were also conducted. vWF bio markers were first found in micro devices to provide initial evidence of AFSC differentiation to endothelial cells. At last, the device and system were successfully established and utilized for its potential of AFSC differentiation to endothelial cells for cardiovascular diseases.
Subjects
microfluidic chip
shear stres
differentiation
endothelial cells
stem cells
SDGs
Type
thesis
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