Microsatellites for the hoopoes (Upupa epops) and their application to the parentage analysis
Resource
CONSERVATION GENETICS RESOURCES, 2(1), 187-189
Journal
Conservation Genetics Resources
Pages
187-189
Date Issued
2010
Date
2010
Author(s)
Hsu, Yu-Cheng
Huang, Chuan-Chin
Severinghaus, Lucia Liu
Abstract
We isolated and characterized nine polymorphic microsatellite loci from a partial genomic library enriched for tetranucleotide repeats in the hoopoe (Upupa epops). A panel of up to 34 adult individuals with unknown relationship was used to characterize polymorphism of these loci. The number of alleles per locus ranged from 5 to 11 and the observed heterozygosity of each locus ranged from 0.250 to 0.853. Only one locus deviated from Hardy-Weinberg equilibrium, with the excess of homozygote. By using five of the nine loci, we conducted paternity analysis on a population of hoopoe on Kinmen Island. The probability of false parent exclusion is 0.984 for the set of loci. In 20 clutches containing parents and juveniles, only one young was sired by extra-pair copulation. In addition to paternity analysis, these microsatellite markers should be useful in population studies for this widely distributed species. © Springer Science+Business Media B.V. 2009.
Type
journal article
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