Glycoprotein B7-H3 overexpression and aberrant glycosylation in oral cancer and immune response
Journal
Proceedings of the National Academy of Sciences of the United States of America
Journal Volume
112
Journal Issue
42
Date Issued
2015-10-20
Author(s)
Chen, Chein-Hung
Ku, Ko-Li
Hsiao, Michael
Hsu, Tsui-Ling
Wong, Chi-Huey
Abstract
The incidence and mortality rate of oral cancer continue to rise, partly due to the lack of effective early diagnosis and increasing environmental exposure to cancer-causing agents. To identify new markers for oral cancer, we used a sialylation probe to investigate the glycoproteins differentially expressed on oral cancer cells. Of the glycoproteins identified, B7 Homolog 3 (B7-H3) was significantly overexpressed in oral squamous cell carcinoma (OSCC), and its overexpression correlated with larger tumor size, advanced clinical stage, and low survival rate in OSCC patients. In addition, knockdown of B7-H3 suppressed tumor cell proliferation, and restoration of B7-H3 expression enhanced tumor growth. It was also found that the N-glycans of B7-H3 from Ca9-22 oral cancer cells contain the terminal α-galactose and are more diverse with higher fucosylation and better interaction with DC-SIGN [DC-specific intercellular adhesion molecule-3 (ICAM-3)-grabbing nonintegrin] and Langerin on immune cells than that from normal cells, suggesting that the glycans on B7-H3 may also play an important role in the disease.
Subjects
CD276; fucose; glycan sequencing; proliferation; terminal α-galactose
CD276; Fucose; Glycan sequencing; Proliferation; Terminal α-galactose
SDGs
Other Subjects
galactose; glycan; glycoprotein; intercellular adhesion molecule 3; langerin; tumor marker; B7 antigen; CD276 protein, human; animal cell; animal experiment; animal model; animal tissue; cancer cell; cancer patient; cancer staging; cell proliferation; Conference Paper; controlled study; fucosylation; gene silencing; glycosylation; immune response; mouse; mouth cancer; mouth squamous cell carcinoma; nonhuman; priority journal; protein expression; sialylation; survival rate; tumor cell; tumor growth; tumor volume; glycosylation; human; immunology; metabolism; mouth tumor; pathology; squamous cell carcinoma; B7 Antigens; Carcinoma, Squamous Cell; Cell Proliferation; Glycosylation; Humans; Mouth Neoplasms
Type
journal article