Establishment and characterization of NS3 protein-specific T-cell clones from a patient with chronic hepatitis C
Journal
Journal of Biomedical Science
Journal Volume
5
Journal Issue
4
Pages
290-296
Date Issued
1998
Author(s)
Hwang L.-H.
Wang J.-M.
Kao S.-F.
Pan C.-H.
Chi W.-K.
Abstract
Our previous study showed dominant proliferative response of peripheral mononuclear cells to hepatitis C virus (HCV) nonstructural (NS-3) (T9, from aa 1188 to 1493) in chronically infected patients. Six T9-specific T-cell clones derived in an HCV patient were established and studied for the antigen specificity and the ability of augmentation of in vitro antibody production. All these cloned T-cell lines responded exclusively to T9 antigen and could help autologous B cells in producing anti-T9 antibody in vitro. Cytokine mRNAs of these T cells was detected by polymerase chain reaction and predominant IL-2 and IFN-γ production was noted. In addition, further elucidation of T-cell antigenic determinant and MHC restriction suggested that these T-cell clones recognized at least two different T-cell antigenic determinants within the NS-3 region in an HLA DQ2-restricted manner. We believe characterization of HCV-specific T-cell responses, especially T-cell epitope mapping and cytokine production pattern, may shed light on further understanding the pathogenic mechanism and designing therapy for HCV infection.
SDGs
Other Subjects
adult; antigenicity; article; case report; cellular immunity; chronic hepatitis; hepatitis C; Hepatitis C virus; human; male; pathogenesis; priority journal; T lymphocyte
Publisher
BioMed Central Ltd.
Type
journal article