Seasonal variation of microbial populations and biomass in Tatachia grassland soils of Taiwan
Resource
Environmental Geochemistry and Health 30 (3): 255-272
Journal
Environmental Geochemistry and Health
Pages
255-272
Date Issued
2008
Date
2008
Author(s)
Cho, Shine-Tsern
Tsai, Shu-Hsien
Ravindran, Anita
Selvam, Ammaiyappan
Yang, Shang-Shyng
Abstract
To investigate the seasonal variations of microbial ecology in grassland of Tatachia forest, soil properties, microbial populations, microbial biomass, and 16S rDNA clone library analysis were determined. The soil had temperatures 6.6-18.4°C, pH 3.6-5.1, total organic carbon 1.11-10.68%, total nitrogen 0.18-0.78%, and C/N ratios 3.46-20.55. Each gram of dry soil contained bacteria, actinomycetes, fungi, cellulolytic, phosphate-solubilizing microbes, and nitrogen-fixing microbes 4.54 × 104 to 3.79 × 107, 3.43 × 102 to 2.17 × 105, 5.74 × 103 to 3.76 × 106, 1.97 × 103 to 1.34 × 106, 8.49 × 102 to 5.59 × 105, and 3.86 × 102 to 3.75 × 105 CFU, respectively. Each gram of soil contained 117-2,482 μg of microbial biomass carbon, 23-216 μg of microbial biomass nitrogen and 9-29 μg of DNA. The microbial populations, microbial biomass, and DNA decreased stepwise with the depth of soil, and they had low values in winter seasons. The microbial populations, microbial biomass carbon, microbial biomass nitrogen, and DNA at the BW2 horizon were 8.42-17.84, 19.26-64.40, 16.84-61.11, and 31.03-46.26% of those at the O horizon, respectively. When analyzing 16S rDNA library, members of Proteobacteria, Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, candidate division TM1, candidate division TM7, Gammatimonadetes, and Verrucomicrobia were identified. Members of Proteobacteria (44.4%) and Acidobacteria (33.3%) dominated the clone libraries. Within the phylum Proteobacteria, α-, β, and γ-Proteobacteria were most numerous, followed by δ-Proteobacteria. © Springer Science+Business Media B.V. 2007.
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