Preparation, Characterization and Application of Chitosan-Pectin Composite Microparticles
Date Issued
2010
Date
2010
Author(s)
Tseng, Yen-Po
Abstract
In this study, chitosan and pectin, two kinds of natural polysaccharide were combined to fabricate chitosan-pectin composite microparticles by emulsion method. The characteristics and potential applications of the composite
microprticles as drug delivery carriers were also investigated.
First, the effects of various operating parameters for emulsion were studied. The results of size analysis showed that the microparticles prepared using surfactant Tween 81 had smaller size. To fix the microparticles, 1 hour of NaOH
treatment time was sufficient without changing the size distributions of microparticles. As the total concentration of disperse phase (chitosan-pectin mixture solutions) increased, the yield of microparticles increased.
The properties were further characterized of chitosan-pectin composite microparticles. The DSC results suggested that the interaction between chitosan and pectin did exist. The results of FTIR implied the presence of the interaction
between amino groups of chitosan and carboxyl groups of pectin. Besides, according to the results of cell culture and MTT assay, the composite microparticles showed almost no cytotoxicity. The water-uptake capacity of composite microparticles increased as the ratio of chitosan to pectin increased. The erosion test showed that the erosion properties of composite mircroparticles were adjustable by altering the composition of microparticles. Finally, using BSA as a model drug, the encapsulation efficiency and release profile of BSA in the microparticles were measured. The results showed that enhanced encapsulation efficiency was achieved by using the composite microparticles. We also found that the more chitosn present in the microparticles, the faster the BSA released under acidic conditions. In contrast, the more pectin present in the microparticles,
the faster the BSA released under basic conditions.
In brief, the chitosan-pectin composite microparticles were prepared by emulsion method. The composite microparticles showed better encapsulation property, and adjustable BSA release profiles. The chitosan-pectin composite microparticles therefore have application potential in controlled release of drugs.
Subjects
Chitosan
Pectin
Composite materials
Microparticles
Emulsion
Controlled release
Type
thesis
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