A Study on Analysis of Biomolecular Recogntion Using a Nanomechanics-based Biosensor
Date Issued
2006
Date
2006
Author(s)
Hwang, Rong-Zhang
DOI
en-US
Abstract
Biomolecular recognition is a natural characteristic in DNA hybridization, protein-protein interaction, DNA-protein interaction, lipid-protein interaction, enzyme-substrate reaction, and cell-ligand binding. Exploring the biomolecular recognition event facilitates the discovery of new drugs, biotechnological methods, and materials for therapeutics and diagnostics. This work has shown antibody-antigen recognition into a direct nanomechanical response of microfabricated cantilever beams. With the bottom-up technology in chemical surface treatment and biomolecular functionality, and the top-down microfabrication on physical devices, the biofilm-induced nanomechanical transduction establishes a biosensor platform in real-time, label-free, and quantitative analysis on biomolecular recognition and thus diagnostics.
First of all, this nanomechanics-based biosensor demonstrates the real-time and in-vitro quantitative detection of disease-related C-reactive protein (CRP). A wide range of clinically relevant CRP concentrations from 1 to 500 µg/mL have been successfully measured with the repeatability of within 7 %, making this biosensor a potential diagnostic technique for inflammatory events or cardiovascular disease. In addition to successful biosensing, sensing surface regeneration is a washing process prior to re-use of biosensors in dissociation of antigen molecules out of anchored antibody. To cater to portability and miniaturization of biosensors in point-of-care applications, the physically electrical regeneration in replacement of highly acidic washing with no additional, sizable containers has been proven on separation of antibody-antigen complexes for nanomechanics-based biosensors. The advantageous feature of the electrical technique over the conventional treatment is evident in long-standing protein activity, providing a relatively high efficiency in dissociation, and miniaturization in entire bioassay system.
Leveraging the mature semiconductor industry technology, this work successfully integrates the microcantilever biosensor with a wireless CMOS device for C-reactive protein detection and electrical regeneration. The entire bioassay system is expected in miniaturization to a size as small as a commercial CD player, and thus to be portable. Therefore, the portable biosensor system allows to be applied for on-site diagnosis to a remote area beyond hospital, for instances, in cases of hurricane and Tsunami. Owing to high compatibility of the microcantilever and standard-CMOS wireless device in terms of process and materials, the System-On-Chip (SOC)-based biosensor is highly expected and potentially miniaturized to a grain size for future implanted health monitoring.
In summary, the nanomechanics-based immunoassay biosensor exhibits its broad applicability, effectiveness, high microelectronics integration, promising wireless sensor networks as well.
Subjects
微懸臂梁生物感測器
生物分子之辨識
蛋白質
Microcantilever-based biosensor
Biomolecular recognition
Protein
SDGs
Type
thesis
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