Explore the Feasibility of Ovariectomy as a Method to Establish Pig Model of Osteoporosis
Date Issued
2014
Date
2014
Author(s)
Wang, Hao
Abstract
Osteoporosis is one of the most common bone disease, according to World Health Organization, 35% of women above 65 year old are suffer for this disease. Although osteoporosis is such a common disease, there is not a safe and effective treatment so far, the commonly used hormone treatment is gradually abandoned because it may raise the potential of breast cancer. On the other hand, America spend ten to fifteen billion dollars on osteoporosis patients every year, so to develop a new effective treatment is very urgent, by that, if we can establish an animal model that simulate human physiology and anatomy better, then the development of osteoporosis treatment would be much easier.
To study the possibility of using ovariectomy as method to establish a porcine osteoporosis model, Binlang pigs were chosen for experimental candidates, BL pig has the same genetic composition as Lanyu pig but white-skinned, and its small size and white skin make it better for clinical model animal.
First experiment was to establishment osteoporosis model of BL pig, nine BL pigs aged ten months were ovariectomied for simulating the menopause of women and develop type 1 osteoporosis. Sixteen months after ovariectomy, dual-energy X-ray absorptiometry (DEXA) were used to measure the bone marrow density (BMD), and compare it with the control pigs to verify the establishment of this model, but the database size is too limited makes the data tough to convert precisely. Secondly, BL pig’s bone marrow-derived mesenchymal stem cells (MSCs) were isolated, tri-linage differentiation and surface antigen detection were conducted to confirm the characteristics of MSC, and verify the possibility of MSC transplantation. In the third experiment, MSC were transplanted to the 6 out of 9 ovariectomied pigs to verify the establishment of this model, and this approach as a treatment for osteoporosis. The results of BMD score scan by DEXA and micro-CT show that have no significant difference (p > 0.05), 2 and 3 months after cell transplantation, respectively. The last experiment is to compare the proliferation and osteogenesis capacity of MSCs isolated from marrow fluid of ovariectomied pigs and healthy Ds-red transgenic pigs. The results reveal that they have no significant difference on colony forming rate, doubling time and osteocyte differentiate ability.
In conclusion, there is not significant bone loss happened on BL pigs ovariectomied for 17 months, and shows no significant effects on Binlang pig bone marrow derived MSC’s proliferation and osteogenic ability. It seems that ovariectomy might not suitable to establish osteoporosis model of BL pig, another ways are worth to consider for establishing pig model of osteoporosis.
Subjects
骨質疏鬆症
賓朗豬
骨髓間葉幹細胞
SDGs
Type
thesis
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