行政院國家科學委員會專題研究計畫期中進度報告:豬第二型環狀病毒單獨感染及混合豬生殖與呼吸綜合症病毒感染對豬隻免疫細胞之影響及其可能之作用機制(2/3)
Date Issued
2005
Date
2005
Author(s)
DOI
932313B002043
Abstract
Porcine circovirus 2 (PCV2) is currently
considered the primary causative agent of
postweaning multisystemic wasting syndrome
(PMWS); however, porcine reproductive and
respiratory syndrome virus (PRRSV) has been
suggested as a co-factor needed for the full
development of PMWS. Both viruses attack
monocyte/macrophage lineage cells and are
suggested immunocompromised. In our previous
study, we have demonstrated the effects of the
infection of both viruses, singular or combined,
on the infectious rate, viability, apoptosis,
phagocytosis and microbial killing function of
swine alveolar macrophages (AMs). We also
have proved the high level of interferon-α
induced by PCV2-infected AMs may cause a
reduction in PRRSV replication and
PRRSV-related AM dysfunction. In present
study, we revealed that the levels of tumor
necrosis alpha (TNF-α), interleukin 8 (IL-8),
alveolar macrophage-derived neutrophil
chemotactic factors II (AMCF-II), granulocyte
colony-stimulating factor (G-CSF), monocyte
chemotactic protein-1 (MCP-1), and a
commonly known apoptotic inducer and also an
inflammatory mediator, Fas ligand (FasL), were
strongly up-regulated in PCV2-infected AMs.
The enhanced expression levels of various
inflammatory cytokines, chemokines and FasL
in PCV2-infected AMs may contribute to the
pathogenesis of interstitial pneumonia in pigs
with PMWS. Moreover, a significant increase in
the expression of FasL and a concurrent
appearance of TNF-α, IL-8, and IFN-α were
observed in PCV2 and PRRSV dually infected
groups as compared with PCV2- or PRRSV-
infected AMs. The result may contribute, at least
partially, to the enhancement of PMWS in
PRRSV co-infected cases and the pathogenesis
of the full development of PMWS clinically.
Subjects
Porcine circovirus 2 (PCV2)
postweaning multisystemic wasting syndrome (PMWS)
porcine reproductive and respiratory
syndrome virus (PRRSV)
syndrome virus (PRRSV)
swine alveolar
macrophages (AMs)
macrophages (AMs)
cytokine
chemokine
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
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