Reciprocal cross-regulation of VND and SND multigene TF families for wood formation in Populus trichocarpa
Journal
Proceedings of the National Academy of Sciences
Journal Volume
114
Journal Issue
45
Pages
E9722
Date Issued
2017
Author(s)
H. Chen
Q. Li
W. Li
J. P. Wang
R. Shi
S. Tunlaya Anukit
P. Shuai
Z. Wang
H. Ma
H. Li
Y. H. Sun
R. R. Sederoff
V. L. Chiang
Abstract
Secondary cell wall (SCW) biosynthesis is the biological process that generates wood, an important renewable feedstock for materials and energy. NAC domain transcription factors, particularly Vascular-Related NAC-Domain (VND) and Secondary Wall-Associated NAC Domain (SND) proteins, are known to regulate SCW differentiation. The regulation of VND and SND is important to maintain homeostasis for plants to avoid abnormal growth and development. We previously identified a splice variant, PtrSND1-A2IR, derived from PtrSND1-A2 as a dominant-negative regulator, which suppresses the transactivation of all PtrSND1 family members. PtrSND1-A2IR also suppresses the self-activation of the PtrSND1 family members except for its cognate transcription factor, PtrSND1-A2, suggesting the existence of an unknown factor needed to regulate PtrSND1-A2. Here, a splice variant, PtrVND6-C1IR, derived from PtrVND6-C1 was discovered that suppresses the protein functions of all PtrVND6 family members. PtrVND6-C1IR also suppresses the expression of all PtrSND1 members, including PtrSND1-A2, demonstrating that PtrVND6-C1IR is the previously unidentified regulator of PtrSND1-A2. We also found that PtrVND6-C1IR cannot suppress the expression of its cognate transcription factor, PtrVND6-C1. PtrVND6-C1 is suppressed by PtrSND1-A2IR. Both PtrVND6-C1IR and PtrSND1-A2IR cannot suppress their cognate transcription factors but can suppress all members of the other family. The results indicate that the splice variants from the PtrVND6 and PtrSND1 family may exert reciprocal cross-regulation for complete transcriptional regulation of these two families in wood formation. This reciprocal cross-regulation between families suggests a general mechanism among NAC domain proteins and likely other transcription factors, where intron-retained splice variants provide an additional level of regulation. © 2017, National Academy of Sciences. All rights reserved.
Subjects
Alternative splicing; NAC transcription factors; Populus trichocarpa; Reciprocal cross-regulation; Wood formation
SDGs
Other Subjects
complementary DNA; plant DNA; plant protein; plant RNA; Ptr secondary wall associated nac domain 1 A2 protein; Ptr secondary wall associated nac domain A2 IR protein; Ptr secondary wall associated nac domain protein1; Ptr vascular related nac domain 6 C1 protein; Ptr vascular related nac domain protein 6; Ptr vascular related nac domain protein 6 C1 IR protein; regulator protein; secondary wall associated nac domain protein; transcription factor; unclassified drug; vascular related nac domain protein; nuclear protein; plant DNA; plant protein; recombinant protein; transcription factor; transcriptome; Article; comparative study; controlled study; differentiation; homeostasis; intron; multigene family; nonhuman; plant development; plant growth; plant tissue; Populus trichocarpa; priority journal; protein DNA interaction; protein domain; protein expression; protein function; protein induction; protein protein interaction; RNA sequence; RNA splicing; transactivation; transcription regulation; wood; alternative RNA splicing; cell wall; gene expression regulation; gene regulatory network; genetics; growth, development and aging; metabolism; molecular cloning; plant gene; plant leaf; Populus; shoot; transcription initiation; wood; xylem; Alternative Splicing; Cell Wall; Cloning, Molecular; DNA, Plant; Gene Expression Regulation, Plant; Gene Regulatory Networks; Genes, Plant; Homeostasis; Multigene Family; Nuclear Proteins; Plant Leaves; Plant Proteins; Plant Shoots; Populus; Recombinant Proteins; Transcription Factors; Transcriptional Activation; Transcriptome; Wood; Xylem
Type
journal article