Dual-functional gold nanoparticles with antimicrobial and proangiogenic activities improve the healing of multidrug-resistant bacteria-infected wounds in diabetic mice
Journal
Biomaterials Science
Journal Volume
7
Journal Issue
11
Pages
4482-4490
Date Issued
2019
Author(s)
Abstract
Gold nanoparticles (Au NPs) are conjugated with the vascular endothelial growth factor-A165 (VEGF-A165) and (11-mercaptoundecyl)-N,N,N-trimethylammonium (11-MTA) cation to form dual-functional gold nanoparticles (11-MTA/VEGF-Au NPs) that possess antimicrobial and proangiogenic activities for wound healing in diabetic (db/db) mice. VEGF-A165 is a popular proangiogenic growth factor that stimulates multiple components in the wound-healing cascade. On the other hand, 11-MTA possesses antibacterial activity and can be bound to Au NPs easily through Au-S bonding. We have found that the surface density of VEGF-A165 plays a vital role in promoting the proliferation, migration, and tube formation of human umbilical vein endothelial cells. 11-MTA tethered on the VEGF-modified Au NPs enables the nanocomposites (i.e., 11-MTA/VEGF-Au NPs) to exhibit a strong antimicrobial activity against multidrug-resistant bacteria [methicillin-resistant S. aureus (MRSA)]. The minimal inhibition concentration of 11-MTA/VEGF-Au NPs is ?450-fold lower than that of 11-MTA, revealing their high antibacterial efficiency. 11-MTA/VEGF-Au NPs exhibit high biocompatibility. 11-MTA/VEGF-Au NPs as dressing materials to treat MRSA-infected wounds in diabetic mice not only show strong in vivo bactericidal activities but also enhance the healing process of the formation of collagen fibers and epithelialization. Our results show that dual-functional 11-MTA/VEGF-Au NPs are promising agents for clinical applications like treating chronic wound infections. ? 2019 The Royal Society of Chemistry.
SDGs
Other Subjects
Bacteria; Biocompatibility; Endothelial cells; Fiber optic sensors; Mammals; Metal nanoparticles; Anti-bacterial activity; Anti-microbial activity; Bactericidal activity; Functional gold nanoparticles; Human umbilical vein endothelial cells; Inhibition concentrations; Proangiogenic activities; Vascular endothelial growth factor; Gold nanoparticles; 11 mercaptoundecyl) n,n,n trimethylammonium; antiinfective agent; gold nanoparticle; quaternary ammonium derivative; unclassified drug; vasculotropin; vasculotropin A165; antiinfective agent; gold; metal nanoparticle; quaternary ammonium derivative; vasculotropin; angiogenesis; animal experiment; animal model; animal tissue; antibacterial activity; Article; cell migration; cell proliferation; chronic wound; collagen fiber; density; epithelization; experimental diabetes mellitus; human; human cell; in vivo study; methicillin resistant Staphylococcus aureus infection; minimum inhibitory concentration; mouse; non insulin dependent diabetes mellitus; nonhuman; priority journal; umbilical vein endothelial cell; wound healing; animal; cell survival; chemistry; drug effect; experimental diabetes mellitus; methicillin resistant Staphylococcus aureus; microbial sensitivity test; microbiology; particle size; pathology; surface property; synthesis; wound healing; wound infection; Animals; Anti-Bacterial Agents; Cell Proliferation; Cell Survival; Diabetes Mellitus, Experimental; Gold; Human Umbilical Vein Endothelial Cells; Humans; Metal Nanoparticles; Methicillin-Resistant Staphylococcus aureus; Mice; Microbial Sensitivity Tests; Particle Size; Quaternary Ammonium Compounds; Surface Properties; Vascular Endothelial Growth Factors; Wound Healing; Wound Infection
Type
journal article