Bioassay-guided Isolation of Active Components from the Fruit of Canarium album toward Inhibition of Cancer Cell Growth
Date Issued
2014
Date
2014
Author(s)
Chang, Yu-Hsiu
Abstract
Chinese olive (Canarium album.), which belongs to family of Burseraceae and genus of Canarium, is a traditional Chinese medicine with antibiotic, anti-virus, anti-inflammatory, as well as detoxification effects, and is used for curing infection, malaria, venom poinsoning. Former results in our lab indicated that the methanol-butanol olive extraction (MBO) significantly repressed the viability of murine colon cancer cells (CT26); however, more investigations for verifying the bioactive compounds in human cells model are needed. In the present study, Chinese olives debris was collected after water extraction, and extracted again with methanol. The methanol extract of Chinese olives was then fractioned by liquid-liquid partition with hexane, ethyl acetate, and butanol. The MBO was collected for further anti-cancer analysis. On the other hand, previous studies in our lab or other lab shows that some phytochemicals increase the activity of enzymes in mitochondria, making mitochondria-dependent viability assays, such as MTT become imprecise. To ascertain the accuracy of methodology, comparison of WST-1 and MTT with the control of cells counting by IN Cell Analyzer 2200 were conducted, and MTT assay was chosen to be the optimal way to determine the cancer cell viability. Results showed that MBO possessed anti-proliferative capacity against several cancer cells including colon cancer cells of HCT116 and HCT116 p53-/-, tongue cancer cell (Ca922), lung cancer cells (A549), and blood cancer cells (HL-60). Besides, MBO didn’t repress the proliferation of normal cell, including Int407 and CaCO2. After processing MBO with acidic buffer solution, MBO-2B fraction possessed enhancing the anti-tumor ability. Furthermore, fractionation the MBO-2B by HP20 resin, obtaining E4 sub-fraction with enriched anti-proliferative effect. By HPLC, compound of Ellagic acid accounted for 72 % of this elution. Hence, Ellagic acid was believed as the functional compound for the antitumor property in Chinese olives.
Subjects
中國橄欖
抑制癌細胞增生
鞣花酸
中國橄欖甲醇萃取物正丁醇區分層
液液萃取
SDGs
Type
thesis
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