Long-Term Intravital Imaging of the Cornea, Skin, and Hair Follicle by Multiphoton Microscope
Journal
Methods in Molecular Biology
Journal Volume
2150
Pages
131-140
Date Issued
2020
Author(s)
Abstract
Multiphoton microscopy allows long-term direct visualization of cells in live animals due to its low photodamage. When coupled with fluorescence protein targeting and second harmonic generation signals from natural collagen as contrast, multiphoton microscopy enables intravital tracing of cells while providing structural information from the extracellular matrix. Compared with conventional histological analysis, it can bring new insight into the cell dynamics in stem cell research. Here, we demonstrate cell imaging and tracing at a single cell resolution in the cornea, skin, and hair follicles using multiphoton microscopy in transgenic mice of which specific cell populations are tagged with fluorescent proteins.
SDGs
Publisher
Humana Press Inc.
Type
book part