Polymorphisms of TNF-α -308 G/A and IL-8 -251 T/A Genes Associated with Urothelial Carcinoma: A Case-Control Study
Journal
BioMed Research International
Journal Volume
2018
Pages
3148137
Date Issued
2018
Author(s)
Abstract
Cigarette smoking and exposure to environmental tobacco smoke are well-known risk factors for urothelial carcinoma (UC). We conducted a hospital-based case-control study involving 287 UC cases and 574 cancer-free controls to investigate the joint effects of cigarette smoking and polymorphisms of inflammatory genes on UC risk. Tumor necrosis factor alpha (TNF-α) -308 G/A and interleukin-8 (IL-8) -251 T/A polymorphisms were determined using a polymerase chain reaction-restriction fragment length polymorphism method. People who had ever smoked and those who were exposed to environmental tobacco smoke had significantly increased UC odds ratios (ORs) of 1.65 and 1.68, respectively. Participants who had smoked more than 18 pack-years had a significantly increased UC OR of 2.64. People who had ever smoked and who carried the A/A genotype of the TNF-α -308 G/A polymorphism had a significantly higher UC OR (10.25) compared to people who had never smoked and who carried the G/G or G/A genotype. In addition, people who had ever smoked and who carried the IL-8 -251 T/T genotype had a significantly increased UC OR (3.08) compared to people who had never smoked and who carried the T/A or A/A genotype. In a combined analysis of three major risk factors (cumulative cigarette smoking, the TNF-α -308 A/A genotype, and the IL-8 -251 T/T genotype), subjects with any one, any two, and all three risk factors experienced significantly increased UC ORs of 1.55, 2.89, and 3.77, respectively, compared to individuals with none of the risk factors. Conclusions. Our results indicate that the combined effects of cumulative cigarette exposure and the TNF-α -308 A/A genotype and/or the IL-8 -251 T/T genotype on UC OR showed a significant dose-response relationship. ? 2018 Chia-Chang Wu et al.
SDGs
Other Subjects
interleukin 8; tumor necrosis factor; interleukin 8; tumor necrosis factor; adult; alcohol consumption; Article; cigarette smoking; comparative study; controlled study; environmental exposure; female; genetic association; genotype; genotype environment interaction; human; major clinical study; male; restriction fragment length polymorphism; sex difference; single nucleotide polymorphism; structured questionnaire; transitional cell carcinoma; aged; case control study; genetic polymorphism; genetic predisposition; genetics; middle aged; risk factor; smoking; Taiwan; transitional cell carcinoma; urinary tract tumor; Adult; Aged; Carcinoma, Transitional Cell; Case-Control Studies; Female; Genetic Predisposition to Disease; Genotype; Humans; Interleukin-8; Male; Middle Aged; Polymorphism, Genetic; Risk Factors; Smoking; Taiwan; Tumor Necrosis Factor-alpha; Urologic Neoplasms
Publisher
Hindawi Limited
Type
journal article