Dynamic modification of the capillary wall for electrophoretic separations of small ions
Resource
Journal of Chromatography A 898 (1): 133-139
Journal
Journal of Chromatography A
Journal Volume
898
Journal Issue
1
Pages
133-139
Date Issued
2000
Date
2000
Author(s)
Abstract
Separations of small ions were carried out under nonequilibrated conditions using capillaries treated with NaOH, HCl, or tris(hydroxymethyl)aminomethane (Tris) prior to analysis. For separations of benzoic acid isomers or acids and amines under weakly acidic conditions, capillaries flushed with 0.1 M NaOH and subsequently with running buffers prior to analysis were used. Separations of six benzoic acid isomers were accomplished in 4 min in 1 mM phosphate buffers, pH 4.01, containing 2.5 mM hydroxypropyl-β-cyclodextrin. Without additives, the separation of biological amines and acids were also achieved in 10 min at pH 4.01. Capillaries treated with 0.1 M HCl prior to analysis were tested in separations of six phenols in 5 mM Tris solutions at pH 7.0. As a result of small electrophoretic mobilities of phenols against a small electroosmotic flow, resolution was optimized. We also found that reproducibility was improved using capillaries treated with HCl. The relative standard deviations of migration mobility of phenols were less than 1%, which were smaller than those obtained using capillaries treated with 0.1 M NaOH or Tris. Copyright (C) 2000 Elsevier Science B.V.
SDGs
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
13.pdf
Size
125.8 KB
Format
Adobe PDF
Checksum
(MD5):49cf9e550596422884546d9ed4aad302