Fabricating microparticles/nanofibers composite and nanofiber scaffold with controllable pore size by rotating multichannel electrospinning
Journal
Journal of Biomaterials Science, Polymer Edition
Journal Volume
21
Journal Issue
11
Pages
1503-1514
Date Issued
2010
Author(s)
Abstract
Polymeric nanofibers fabricated via electrospinning are regarded as promising scaffolds for biomimicking a native extracellular matrix. However, electrospun scaffolds have poor porosity, resulting in cells being unable to infiltrate into the scaffolds but grow only on its surface. In this study, we modified regular electrospinning into rotating multichannel electrospinning (RM-ELSP) to produce microparticles and nanofibers simultaneously. Gelatin nanofibers (0.1-1 μm) and polycaprolactone (PCL) microparticles (0.5-10 μm) were formed and well-mixed. Adjusting the concentration of PCL and/or gelatin, we can fabricate various microparticles/nanofibers composites with different sizes of PCL particles and different diameters of gelatin nanofibers depending on their concentrations (2-10%) during electrospinning. Using PCL particles as a pore generator, we obtained gelatin nanofiber scaffolds with controllable pore size and porosity. Cells adhere and grow into the scaffold easily during in vitro cell culture. ? 2010 Koninklijke Brill NV, Leiden.
Subjects
electrospinning; gelatin; Microenvironment; nanofiber scaffold; polycaprolactone
SDGs
Other Subjects
Cell culture; Electrospinning; Fabrication; Nanofibers; Polycaprolactone; Pore size; Electrospun scaffolds; gelatin; Gelatin nanofibers; Micro-particles; Microenvironments; Nanofiber scaffold; Native extracellular matrix; Polymeric nanofibers; Scaffolds (biology); gelatin; molecular scaffold; nanocomposite; nanofiber; polycaprolactone; biomaterial; nanocomposite; nanofiber; polyester; animal cell; article; cell adhesion; cell culture; cell proliferation; cell structure; composite material; cross linking; cytotoxicity; electron microscopy; electrospinning; fibroblast; in vitro study; microenvironment; mouse; nanofabrication; nonhuman; porosity; priority journal; animal; cell survival; chemistry; drug effects; Institute for Cancer Research mouse; nanotechnology; porosity; procedures; scanning electron microscopy; synthesis; tissue scaffold; Animals; Biocompatible Materials; Cell Proliferation; Cell Survival; Cells, Cultured; Gelatin; Mice; Mice, Inbred ICR; Microscopy, Electron, Scanning; Nanocomposites; Nanofibers; Nanotechnology; Polyesters; Porosity; Tissue Scaffolds
Type
journal article