Development of a Calcium Phosphate Cement with γ-PGA/ Gelatin Hydrogel Carrying TGF-β1 for Vital Pulp Therapy
Date Issued
2015
Date
2015
Author(s)
Wang, Yu-Hsiang
Abstract
Vital pulp therapy is to preserve the pulp vitality as well as to form the reparative dentin for maintaining the normal physical structures and functions of teeth. After years of research and development of the biomaterials, there is no ideal material for vital pulp therapy. Bioactive calcium phosphate cements (CPCs) have been widely used for repairing bone defects because of their excellent biocompatibility and bioactivity. However, CPCs only demonstrated comparative effect with commercial MTA in studies in vital pulp therapy. In this study we evaluated the alpha-tricalcium phosphate cement (α-TCP) with γ-PGA/ gelatin hydrogel carrying TGF-β1 application on vital pulp therapy. Poly-γ-glumatic acid (γ-PGA) is suitable candidate for drug carrier. And TGF-β1 can induce differentiation of dental pulp cell. The purpose of this study is to evaluate effect of CPC/ hydrogel/ TGF-β1 on pulp-dentin regeneration and vital pulp therapy. We examined the possible intervening effects of hydrogel on the clinical compliance of CPC with assessment of its setting time, compressive strength, degradation, crystallinity, and microscopic structure. Biocompatibility, in vitro bioactivity on cultured human dental pulp cell was evaluated and also animal study was performed to evaluate the ability of CPC/ hydrogel/ TGF-β1 to induce hard tissue formation in vivo. The results showed that the setting time and compressive strength of CPC/ hydrogel exhibited no significant difference compared with CPC. The cell viability and cytotoxicity exhibited no harm to the dental pulp cell and animal study presents this CPC/ hydrogel/ TGF-β1 can induce calcified tissue formation. Based on the results, the developed CPC/ hydrogel/ TGF-β1 has great potential as a material for vital pulp therapy.
Subjects
calcium phosphate cements
alpha-tricalcium phosphate
vital pulp therapy
Type
thesis
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