IGZO-TFT Protein Sensors with ZnO nanorods for Enhanced Sensitivity and Specificity
Date Issued
2014
Date
2014
Author(s)
Shen, Yi-Chun
Abstract
In this thesis, tumor related assays are introduced based on the properties of ZnO nanorods. ZnO nanorods are able to form biomarkers of specificity together with antibodies. EGFR antibody is selected since it specifically binds to EGFR protein which is well-known to be overexpressed in cancer cells. ZnO nanorods/EGFR antibody probes bind to cancer cells through EGFR and emit purple light around 377nm as being excited by the UV luminance. By photoluminescent spectrum and image, cancer cells can be differentiated. The method prevents the photobleaching happened in traditional fluorescent detection and achieves the real-time detection.
ZnO nanorods are also applied to IGZO-TFT protein biosensor to improve the sensitivity of detection. The bio-TFT is of extended gold pad to enlarge the sensing area and isolate the biochemical solutions. ZnO nanorods and EGFR antibody are used to functionalize the sensing pad for specific detection of EGFR. The biocompatibility and high surface-to-volume ratio of ZnO nanorods improve the sensitivity of the device significantly. By this novel structure, a time-saving and highly sensitive protein detecting process is developed. The bio-TFT is able to selectively detecting 36.2 fM of EGFR in the total protein solution of 0.1 ng/ml.
Subjects
癌症
表皮生長因子接受器
氧化鋅奈米柱
高敏感度生物感測器
SDGs
Type
thesis
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