Molecular Analysis of the Taiwanese Patients with Hypertriglyceridemia and Expression of the Associated Apolipoprotein
Date Issued
2009
Date
2009
Author(s)
Chen, Yen-Hsin
Abstract
The differences in eating habits and less exercise influence people’s health condition. The increasing people suffered from metabolic syndrome including Hypertriglyceridemia (HTG) which results from genetic and environmental factors. Some researches say that the elevated triglycerides (TGs) are related to coronary artery disease (CAD) and cerebrovascular disease. We supposed to do is consider what caused HTG and clarify the interaction of genotypes. We collect three groups of Controls (TGs<2.26mmol/L, n=285), Moderate HTG (2.26≦TGs<5.65 mmol/L, n=216), and Severe HTG (TGs≧5.65mmol/L, n=169) from Dr. Su’s metabolic clinic in National Taiwan University Hospital. Get information of their age, BMI, waist, smoking habit and the history of hypertension associated with HTG patients, and identify their significance in statistics (p-value<0.0001). And then consider the biochemical analysis, we find the expressions of cholesterol (CHO), TGs, Glucose AC, Insulin AC in severe HTG are higher than moderate HTG and Controls (p-valus<0.0001). According to previous papers, we pick up three candidate gene (APOA5, APOE and APOC3) to do genetic analysis. If we find the 553G>T polymorphism in APOA5 gene, apolipoprotin A5 cannot modulate the transport of TGs and cause TGs increase. The replacement of C to G occurred in 3’ untranslated region at 3238 site of APOC3 gene, which make apolipoprotein C3 fail to regulate TGs metabolism. The consequence is TGs elevated. And if the genotype is APOE4, the function of TGs transport and metabolism will not work well and the concentration of TGs will be high. There are statistic significances (p-value<0.0001) in high ratio of T allele in APOA5 and APOE4 allele. Consequently, APOA5, APOE4 and APOC3 may be related to HTG. Therefore we use univariate and multivariate logistic regression analysis to analyze the interaction between these three genes. We postulate the co-existence of apoliprotein A5 and apolipoprotein E4 influence the function of TGs regulation and then TGs increase. Otherwise, we also observe the protein level of apolipoprotein E4, C3 and A5. Then, we find that the concentration of apolipoprotein C3 is associated with lipid profile (p-value<0.0001). As a result, we assume that the level of apolipoprotein C3 is associated with TGs level in HTG patients. Clarify APOA5, APOE4, and APOC3 are associated with HTG. What we should do is suggest the patients with HTG do genetic analysis to confirm genotype, and then help them change their life style to control TGs level and avoid coronary artery disease.
Subjects
hypertriglyceridemia (HTG)
triglycerides (TGs)
coronary artery disease (CAD)
cerebrovascular disease
candidate gene
APOA5
APOC3
APOE4
polymorphism
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