Dioscorea Phytocompounds Enhance Murine Splenocyte Proliferation Ex Vivo and Improve Regeneration of Bone Marrow Cells In Vivo
Resource
eCAM
Journal
Evidence-based Complementary and Alternative Medicine
Pages
1
Date Issued
2010
Date
2010
Author(s)
Su, Pei-Fen
Li, Chin-Jin
Hsu, Chih-Chien
Benson, Spencer
Wang, Sheng-Yang
Aravindaram, Kandan
Chan, Sunney I.
Wu, Shih-Hsiung
Yang, Feng-Ling
Huang, Wen-Ching
Shyur, Lie-Fen
Yang, Ning-Sun
Abstract
Specific cytokines have been tested clinically for immunotherapy of cancers; however, cytotoxicity has often impaired their usefulness. Consequently, alternative approaches are increasingly desirable. Dioscorea spp. tuber is a widely used traditional Chinese medicinal herb claimed to confer immunostimulatory activity. In this study, we evaluated Dioscorea as an adjuvant therapy for use alongside chemotherapy for cancer. Phytocompounds from Dioscorea tubers were ethanol fractioned and used for ex vivo splenocyte proliferation assay or in vivo force-feeding of mice pre-treated with the chemotherapy agent 5-fluorouracil. Co-treatment with a 50-75 ethanol-partitioned fraction of the tuber extract of D. batatas (DsCE-II) and interleukin (IL)-2 resulted in a significantly higher rate of murine splenocyte cell proliferation ex vivo than treatment with DsCE-II or IL-2 alone. This DsCE-II fraction, which contains a polysaccharide with a high proportion of β-1,4-linkage mannose (?64), also promoted the regeneration of specific progenitor cell populations in damaged bone marrow tissues of 5-fluorouracil-treated mice. Colony-forming unit (CFU) analyses demonstrated that the population of CFU-GM cells, but not CFU-GEMM or BFU-E cells, preferentially recovered to 67 in the bone marrow of immune-suppressed mice fed with DsCE-II. DsCE-II efficacy level was ?85 of that obtained by subcutaneous administration of recombinant G-CSF proteins (5μgkg-1) in mice tested in parallel. This study suggests that the DsCE-II fraction of D. batatas extract may be considered for further development as a dietary supplement for use alongside chemotherapy during cancer treatment. Copyright ? 2011 Pei-Fen Su et al.
SDGs
Other Subjects
alcohol; Dioscorea phytocompound; fluorouracil; interleukin 2; mannose; plant medicinal product; polysaccharide; unclassified drug; animal cell; animal experiment; article; bone marrow cell; cancer chemotherapy; cell assay; cell proliferation; cell regeneration; colony forming unit; controlled study; ex vivo study; female; in vivo study; mouse; nonhuman; priority journal; spleen cell; yam
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