The role of Kruppel-like Factor 4 in Canine Mammary Gland Tumor Cells
Date Issued
2012
Date
2012
Author(s)
Tien, Yung-Tien
Abstract
Canine mammary gland tumor (cMGT) is the most common tumor happened in female dogs and more than 50% of cases were diagnosed as malignant cancers. Though the high mobility and malignancy of MGT are recognized, there are still no effect treatments to elongate the disease-free survival time. Kruppel-like factor 4 (KLF4) is a transcription factor of KLF family and related to cell proliferation, differentiation, migration and apoptosis. Also, KLF4 could induce pluripotent stem cell from differentiated somatic cells with other defined factor. In this study, we identified 844 bp of canine Klf4 sequence from blasting the mRNA of mouse, rat and human and the result was used to designed specific primer pair to detect the Klf4 expression in MGT cells. On the other hand, we detected KLF4 protein expression in normal canine tissue and MGT cells by using polyclonal antibody. We identified KLF4 would abundantly expression in normal canine intestine, skin epithelium and MGT cell lines. In order to realize the role of KLF4 in MGT cells, we downregulated KLF4 expression with Ken and the results suggested Ken would downregulated KLF4 expression in transcriptional and translational level after 8 to 24 hours incubation. Also, the biofunciton assays were tested in Ken-treated MGT cells and the results shown MGT cells would lose the ability of in vitro tumorigenicity and characters of malignancy.
Subjects
canine mammary gland tumor (cMGT)
SDGs
Type
thesis
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