The characteristics of Lymantria dispar sub-cloned cell strains IPLB-LD-652Y-a~f and the susceptibility to LyxyMNPV
Date Issued
2008
Date
2008
Author(s)
Yang, Yi-Ting
Abstract
Insect in vitro culture system has been developed for a century and wild applied in study of baculovirus and the recombinant protein production.The gypsy moth (Lymantria dispar) cell line, IPLB-LD-652Y, was subcloned six cell strains, IPLB-LD-652Y-a~f (LD-a ~ -f). These cell strains contain different proportions of four morphological different cells: round, spindle, squamous, and polymorphous cells. LD-a cells contain more spindle-shaped cells. The predominant cells in LD-d cells are elongate spindle cells (sensory neurve-like cells), and in LD-b, -c and -f cells are round cells. The growth rates of cell strains were faster than parental cells. These cell strains showed the same esterase isozyme pattern with that of parental cell line. LD-b and -f cells contained more lipid droplets in cytoplasm than other cell strains, while the lipid droplets were dispersed evenly in LD-b cells and aggregated in LD-f cells. All the cell strains and the parental cells were highly susceptible to LyxyMNPV-5 and LyxyNPV-2, which were isolated from the moribund larvae of L. xylina with nucleopolyhedrosis, the average infection rate was almost 98% susceptible to the viruses at 14 dpi. LD-a had high yields of polyhedra, but lower virus titer of ECV among cell strains. And the cell strains also showed a high susceptibility to fluorescent recombinant viruses, LyxyExp-EGFP and LyxyExp-DsRed. LD-a cells produced the highest amount of DsRed and EGFP, respectively, but LD-d produced the lowest recombinant proteins. The results of this study showed that sub-cloned cell strains had different morphology, characteristics and virus susceptibility compared with parental cells. We conclude that LD-a cells will be potentially useful for producing recombinant proteins.
Subjects
Lymantria dispar
cell strain
LyxyMNPV
Type
thesis
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