BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing
Journal
RNA
Journal Volume
19
Journal Issue
2
Pages
208-218
Date Issued
2013
Author(s)
Chen P.-H.
Lee C.-I.
Weng Y.-T.
Tarn W.-Y.
Tsao Y.-P.
Kuo P.-C.
Hsu P.-H.
Huang C.-W.
Wu J.-T.
Abstract
Here, we show that dBCAS2 (CG4980, human Breast Carcinoma Amplified Sequence 2 ortholog) is essential for the viability of Drosophila melanogaster. We find that ubiquitous or tissue-specific depletion of dBCAS2 leads to larval lethality, wing deformities, impaired splicing, and apoptosis. More importantly, overexpression of hBCAS2 rescues these defects. Furthermore, the C-terminal coiled-coil domain of hBCAS2 binds directly to CDC5L and recruits hPrp19/PLRG1 to form a core complex for splicing in mammalian cells and can partially restore wing damage induced by knocking down dBCAS2 in flies. In summary, Drosophila and human BCAS2 share a similar function in RNA splicing, which affects cell viability.
Type
journal article