Activation of NK cell cytotoxicity by the natural compound 2,3-butanediol
Journal
Journal of Leukocyte Biology
Journal Volume
92
Journal Issue
4
Pages
807-814
Date Issued
2012
Author(s)
Chang C.-J.
Yang C.-H.
Hsu Y.-J.
Chen C.-C.
Lin C.-S.
Tsai Y.-H.
Huang T.-T.
Ojcius D.M.
Tsai Y.-H.
Lu C.-C.
Abstract
The natural compound 2,3-BTD has diverse physiological effects in a range of organisms, including acting as a detoxifying product of liver alcohol metabolism in humans and ameliorating endotoxin-induced acute lung injury in rats. In this study, we reveal that 2,3-BTD enhances NK cell cytotoxic activity in human pNK cells and NK92 cells. Treatment of NK cells with 2,3-BTD increased perforin expression in a dose-dependent manner. This was accompanied by elevated JNK and ERK1/2 MAPK activities and enhanced expression of NKG2D/NCRs, upstream signaling molecules of the MAPK pathways. The 2,3-BTD effect was inhibited by pretreatment with inhibitors of JNK (SP) or ERK1/2 (PD) or by depleting NKG2D/NCRs or JNK1 or ERK2 with siRNA. These results indicate that 2,3-BTD activates NK cell cytotoxicity by NKG2D/NCR pathways and represent the first report of the 2,3-BTD effect on activation of innate immunity cells.
SDGs
Type
journal article