Assessment of Candida Glabrata Strain Relatedness by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing
Resource
JOURNAL OF CLINICAL MICROBIOLOGY v.45 n.8 pp.2452-2459
Journal
JOURNAL OF CLINICAL MICROBIOLOGY
Journal Volume
v.45
Journal Issue
n.8
Pages
2452-2459
Date Issued
2007
Date
2007
Author(s)
CHEN, YEE-CHUN
Abstract
In this study, 80 Candida glabrata isolates from intensive care unit and human immunodeficiency virus (HIV)-infected patients were typed by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) , and mating type class determination. Among the 25 patients with multiple isolates, 19 patients (76%) contained multiple isolates exhibiting identical or highly related PFGE and MLST genotypes, which may indicate the maintenance or microvariation of one C. glabrata strain in each patient. However, isolates from six patients (24%) displayed different sequence types, PFGE genotypes, or mating type classes, which may indicate colonization with more than one clone over time or strain replacement. High correlations among PFGE genotypes, sequence types, and mating types were found (P < 0.01). MLST exhibited less discriminatory power than PFGE with BssHII. The genotypes, sequence types, and mating type classes were independent of anatomic sources, drug susceptibility, and HIV infection status.
Subjects
BLOOD-STREAM INFECTIONS
IN-VITRO SUSCEPTIBILITIES
CRITICALLY ILL PATIENTS
ALBICANS STRAINS
MOLECULAR EPIDEMIOLOGY
NOSOCOMIAL CANDIDEMIA
SDGs