A simple method for the construction of a recording-injection microelectrode with glass-insulated microwire
Resource
Journal of Neuroscience Methods 72 (1): 1-4
Journal
Journal of Neuroscience Methods
Journal Volume
72
Journal Issue
1
Pages
1-4
Date Issued
1997
Date
1997
Author(s)
Abstract
A rapid method for the production of a glass-insulated microwire electrode is described. A microwire was threaded into a glass capillary which was then pulled on a vertical pipette puller. A conical tip of the microwire was formed when the strongly heated glass capillary broke together with the wire in it. A tight seal of the glass-insulated microwire electrode between the glass and the metal was accomplished with silicone glue. The manufactured electrode performed consistently at different immersion depths, and yielded stable recordings of single units in the cerebral cortex and the medulla of rats. The strength and low impedance characteristics of the glass-insulated microwire electrode may make it useful for the recording of single units in deep brain structures. Furthermore, the electrode can be easily combined with another glass micropipette to form a dual recording-injection microelectrode unit.
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