The Observation of Interaction Between Pseudomonas aeruginosa and Cornea by the Use of Two-Photon Fluorescence and Second Harmonic Generation Microscopy
Date Issued
2007
Date
2007
Author(s)
Chang, Yu-Lin
DOI
zh-TW
Abstract
The eyes are the most direct sensory organ in our body to receive and explore the world, and the corneas residing in the outmost frontline easily result in suffering invasion and destruction by external matter, lead to keratitis, affect vision ability and lose vision seriously. In the generalization of contact lens develops day by day, on the issue associated with corneal infection due to incorrect habit of contact lens is put much emphasis. Pseudomonas aeruginosa is one of the most important infectious pathogens, it will adhere on the cornea and destroy the corneal tissue opportunistically with its quite violent virulence. The infected corneas will be attacked to necrosis by one to two days with very fast destructive speed. It is very important to diagnosis the infectious pathogen in time. We can investigate the morphology and the structure of the corneal epithelium and stroma collagen by the use of minimally invasive two-photon fluorescence and second harmonic generation microscopy. We culture the bovine cornea as our specimens, and inject into each specimen with bacteria suspension or PBS, and image the region nearby the injection hole of each cornea at different time. As the time went by, there are more increase in the severity of the stromal collagen destruction, the more outspreading pattern and proliferation occurred in bacteria activity, and more abundant substance with auto-fluorescence within the cornea. For our controls, there are a little decay of the signals of stromal collagen, and no emergence of the huge auto-fluorescence signals. We can make the morphological discuss via these preliminary experiment, and as for the quality, quantity analysis, and the fixed point observation should need the refinement of the experimental methods. We expect that this multi-photon microscopy could apply in clinic in the future, to assist doctors in diagnosing the degree of corneal infection and treating in time without destructive histology biopsy.
Subjects
雙光子螢光
二倍頻
顯微術
角膜
感染
綠膿桿菌
two-photon fluorescence
second harmonic generation
microscopy
cornea
infection
Pseudomonas aeruginosa
Type
thesis
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