Fluorescent nanodiamond - A novel nanomaterial for in vivo applications
Journal
Materials Research Society Symposium Proceedings
Journal Volume
1362
Pages
25 - 35
Date Issued
2011
Author(s)
Mohan, Nitin
Zhang, Bailin
Chang, Cheng-Chun
Yang, Liling
Chen, Chao-Sheng
Fang, Chia-Yi
Hsieh, Hsiao-Han
Cho, Chun-Yi
Weng, Jui-Hsia
Chung, Bon-Chu
Chang, Huan-Cheng
Abstract
ABSTRACTFluorescent nanodiamonds (FNDs) with a size in the range of 10 �V 100 nm have been produced by ion irradiation and annealing, and isolated by differential centrifugation. Single particle spectroscopic characterization with confocal fluorescence microscopy and fluorescence correlation spectroscopy indicates that they are photostable and useful as an alternative to far-red fluorescent proteins for bioimaging applications. We demonstrate the application by performingin vivoimaging of bare and bioconjugated FND particles (100 nm in diameter) inC. elegansand zebrafishes and exploring the interactions between this novel nanomaterial and the model organisms. Our results indicate that FNDs can be delivered to the embryos of both organisms by microinjection and eventually into the hatched larvae in the next generation. No deleterious effects have been observed for the carbon-based nanoparticlesin vivo. The high fluorescence brightness, excellent photostability, and nontoxic nature of the nanomaterial have allowed long-term imaging and tracking of embryogenesis in the organisms.
Event(s)
2011 MRS Spring Meeting
Type
conference paper