P2X3-mediated peripheral sensitization of neuropathic pain in resiniferatoxin-induced neuropathy
Journal
Experimental Neurology
Journal Volume
235
Journal Issue
1
Pages
316-325
Date Issued
2012
Author(s)
Abstract
Patients suffering from sensory neuropathy due to skin denervation frequently have paradoxical manifestations of reduced nociception and neuropathic pain. However, there is a lack of satisfactory animal models to investigate these phenomena and underlying mechanisms. We developed a mouse system of neuropathy induced by resiniferatoxin (RTX), a capsaicin analog, and examined the functional significance of P2X3 receptor in neuropathic pain. From day 7 of RTX neuropathy, mice displayed mechanical allodynia (p< 0.0001) and thermal hypoalgesia (p< 0.0001). After RTX treatment, dorsal root ganglion (DRG) neurons of the peripherin type were depleted (p= 0.012), while neurofilament (+) DRG neurons were not affected (p= 0.62). In addition, RTX caused a shift in neuronal profiles of DRG: (1) increased in P2X3 receptor (p= 0.0002) and ATF3 (p= 0.0006) but (2) reduced TRPV1 (p= 0.036) and CGRP (p= 0.015). The number of P2X3(+)/ATF3(+) neurons was linearly correlated with mechanical thresholds (p= 0.0017). The peripheral expression of P2X3 receptor in dermal nerves was accordingly increased (p= 0.016), and an intraplantar injection of the P2X3 antagonists, A-317491 and TNP-ATP, relieved mechanical allodynia in a dose-dependent manner. In conclusion, RTX-induced sensory neuropathy with upregulation of P2X3 receptor for peripheral sensitization of mechanical allodynia, which provides a new therapeutic target for neuropathic pain after skin denervation. © 2012.
SDGs
Type
journal article