克弗爾應用於新美容產品之開發研究
Date Issued
2005
Date
2005
Author(s)
林慶文
DOI
932313B002099
Abstract
克弗爾為一種由乳酸菌及酵母菌共同發酵之酒精發酵乳，本研究旨在探討克弗爾中之不同成分，對於美白、抗氧化及痤瘡治療是否具有功效，以提升畜產品之應用價值。於美白試驗中，主要以酪胺酸酶之抑制活性來做評估。黑色素主要由酪胺酸藉由酪胺酸酶之催化，經過一連串之反應所形成，故減少黑色素的形成可藉由抑制酪胺酸酶活性達成。結果顯示，乳酸在8mg/mL的添加下，於10 分鐘後之黑色素生成可達IC50，濃度提升為10mg/mL時，酪胺酸酶已不具活性。証據顯示自由基的形成對於皮膚老化有顯著影響，故本研究以銅離子螯合能力做為克弗爾抗氧化能力
之指標，推測克弗爾是否具有抗老化之功能。結果發現乳清、肽類、醣類、菌之胞內萃取物均對銅離子產生螯合能力，顯示克弗爾具有明顯之抗氧化活性。對於痤瘡改善，乳酸於濃度為60mg/mL及10mg/mL分別對痤瘡桿菌及表皮葡萄球菌產生抑制能力，相較於此二株菌對酸環境及抗生素耐受性之不同，或許可供治療炎性痤瘡之參考。
Subjects
Kefir is a cultured milk beverage
produced by microbial action. The purpose of
this research is to study the effects of
different kefir components on skin properties
including skin whitening effect, antioxidative
activity and acne treatment. The final aim is
enhance the value of dairy products. The skin
whitening tests were performed by tyrosinase
assay. The melanin synthesis is regulated by
tyrosinase, which catalyzes the conversions
of tyrosine to dopa and dopa to dopaquinone.
Inhibition of tyrosinase activity reduced the
melanin production. The results showed that
lactic acid had better inhibitory ability agaist
melanin synthesis. A vast amount of evidence
implicates oxygen-derived free radicals as
important causative agents of aging.
Therefore, the antioxidative activities of kefir
components were determined by cupric ion
chelating analysis and results demonstrated
that certain kefir components had
antioxdative activities. The ability of acne
treatment was performed by inhibition of
Propionibacterium acne and Staphylococcus
epidermidis, which commonly isolated from
pustular acne lesions. The results indicated
that lactic acid level higher than 60 and
2
10mg/mL could inhibit the growth of
Propionibacterium acne and Staphylococcus
epidermidis, individually, while no inhibition
was found for other components.
produced by microbial action. The purpose of
this research is to study the effects of
different kefir components on skin properties
including skin whitening effect, antioxidative
activity and acne treatment. The final aim is
enhance the value of dairy products. The skin
whitening tests were performed by tyrosinase
assay. The melanin synthesis is regulated by
tyrosinase, which catalyzes the conversions
of tyrosine to dopa and dopa to dopaquinone.
Inhibition of tyrosinase activity reduced the
melanin production. The results showed that
lactic acid had better inhibitory ability agaist
melanin synthesis. A vast amount of evidence
implicates oxygen-derived free radicals as
important causative agents of aging.
Therefore, the antioxidative activities of kefir
components were determined by cupric ion
chelating analysis and results demonstrated
that certain kefir components had
antioxdative activities. The ability of acne
treatment was performed by inhibition of
Propionibacterium acne and Staphylococcus
epidermidis, which commonly isolated from
pustular acne lesions. The results indicated
that lactic acid level higher than 60 and
2
10mg/mL could inhibit the growth of
Propionibacterium acne and Staphylococcus
epidermidis, individually, while no inhibition
was found for other components.
Publisher
臺北市：國立臺灣大學動物科學技術學系暨研究所
Type
report
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