葉酸對產蛋雞卵巢濾泡生長,類固醇生成,產蛋性能和蛋品質之影響(1/2)
Date Issued
2003
Date
2003
Author(s)
DOI
912313B002389
Abstract
The objectives of this two-year studies is to investigate the effects of active
folate on:(1)ovarian steroidogenesis of ovarian follicular cell.(2)egg productive
performance and egg quality of laying hen.
The first year, the ovarian follicles of laying hen are cultured in vitro. Granulosa
cells taken from large ovarian follicular cells(F1)were stimulated by folates and
gonadotropin, such as oLH、8-bromo-cAMP to evaluate the ability of progesterone
production. The preliminary results showed that two types of folate-Na2FA 與CaFA
could not change the progesterone secretion from granulosa cells in vitro.
In combine with oLH trial, addition of 0.1~1 ug/ml CaFA could inhibited
the progesterone secretion from granulosa cells, but did not observe in
Na2FA treatment. In combine with 100 uM cAMP trial, CaFA decreased the
progesterone production from granulosa cells with increasing CaFA dosage.
This phenomenon did not show in Na2FA treatment. This difference in
progesterone production between CaFA and Na2FA treatments is required further
study.
Subjects
Folate
ovarian follicle
progesterone
Publisher
臺北市:國立臺灣大學動物科學技術學系暨研究所
Type
report
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