The effects of peanut stilbenoids on HL-60 cell line
Date Issued
2008
Date
2008
Author(s)
Abstract
Stilbenoids are phytoalexins produced by plants under attack by pathogens such as fungi. Previous sutdies have demonstrated that stilbenoids have bioactivities such as anti-oxidation, anti-inflammation, cancer chemoprevention and anti-carcinogenic effect. In this study, resveratrol (RSV), arachidin-1 (Ara-1), arachidin-3 (Ara-3), and isopentadienylresveratrol (IPD) were isolated from peanuts. RSV, Ara-1 and Ara-3 display cytotoxic effect on HL-60, and Ara-1 is the most toxic. Further study showed that Ara-1 treatment on HL-60 cells caused loss of mitochondrial membrane potential (MMP), caspases activation, and changes in cell cycle distribution. Ara-3 treatment on HL-60 cells caused loss of MMP and cell membrane integrity as well as caspases activation. These results suggested that Ara-1 and Ara-3 are potential anticancer agents that induced cancer cell apoptosis. Ara-1 and RSV are cytotoxic to proliferating cells, HL-60, but did not affect on nonproliferating human peripheral blood mononuclear cells. Furthermore, Ara-1 was found to inhibit the activity of topoisomerase II in vitro, but showed no significant difference in cytotoxicity between HL-60 and Topo II defective HL-60/MX2 cells. Topoisomerase II inhibition may not be involved in the mechanisms of apoptosis induction by Ara-1 in HL-60 cells. These results indicate that Ara-1 and Ara-3 are potential anti-cancer agents.
Subjects
stilbenoids
Apoptosis
Topoisomerase II
SDGs
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