Activation of hepatic oval cell after ligation of major hepatic vascular inflow in mice
Date Issued
2001-07-31
Date
2001-07-31
Author(s)
袁瑞晃
DOI
892314B002436
Abstract
Liver is a very important visceral organ
for the life. In the oriental country, especially
in China and Taiwan, hepatic disease
including hepatitis and liver cirrhosis is
common and plays an important role in the
health problem. For patients with end-staged
liver disease, nothing can be done except
liver transplantation. It’s not easy to have a
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donor suitable for the recipient from time to
time and further new ways for this problem is
required in the near future.
The liver is usually able to mount a
prompt proliferative response to parenchymal
loss and other hyperplastic stimuli. Following
70% partial hepatectomy in the rat,
hepatocyte proliferation is greatly accelerated
for a relatively short period while the deficit
in hepatocyte number is replaced. Even
though primary culture of the hepatocytes
had been done for years the proliferative
ability and viability decreased after several
generations. So further researches for the
hepatic stem cells that can be cultured for a
long period and have the ability to
differentiate to mature hepatocytes become
more and more important. The principle in
animal model is that when majority of the
hepatic parenchyma is damaged, the ability
of surviving hepatocytes can be regenerated
quickly without activation of hepatic stem
cells (oval cells). Further hepatic insult that
inhibits hepatocyte regeneration is required
for activation of oval cells.
Acetylaminofluorene (AAF), an aromatic
amine, can be metabolized in hepatocytes and
resulted in N-hydroxy derivatives which was
cytotoxic and mitoinhibitory for hepatocytes
but not biliary cells or oval cells. The
purpose of this study is to evaluate the
additional effect of major hepatic vascular
ligation (ligation of the vascular inflow to the
middle and left lobes and preserve the right
lobe only, that equal to about 70% partial
hepatectomy in our preliminary study) and
hepatotoxic agent administration in
activation of hepatic oval cells.
Animals were randomly assigned to four
different groups. Mice receiving vascular
occlusion only (V-group); mice receiving low
dose (5mg/kg) of AAF and vascular ligation
(AV-L-group); mice receiving high dose
(7.5mg/kg) of AAF and vascular ligation
(AV-H-group); and control group (C-group).
AAF was given continuously until the mouse
was sacrificed in all groups. After various
time intervals (3, 5, 7, 9, 14 days)
postoperatively, the mice were sacrificed and
the liver was taken for further studies.
BrdU incorporation with BrdU-FLUOS
kit was used for regeneration evaluation and
immunohistochemical studies about the
cytokeratin18, cytokeratin19, cytokeratin7,
Thy-1, were used to evaluate the activation of
hepatic oval cell.
Subjects
hepatic vascular inflow ligation
hepatic oval cell
SDGs
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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