Calreticulin activates β1 integrin via fucosylation by fucosyltransferase 1 in J82 human bladder cancer cells
Journal
Biochemical Journal
Journal Volume
460
Journal Issue
1
Pages
69-78
Date Issued
2014
Author(s)
Lu, Y.-C.
Chu C.-Y.
Lu J.H.
Wang B.-J.
Chen C.-H.
Lin T.-H.
Pan C.-C.
Chen S.-S.A.
Liao Y.-F.
Wu P.-Y.
Hsia H.-Y.
Chang C.-C.
Abstract
Fucosylation regulates various pathological events in cells. We reported that different levels of CRT (calreticulin) affect the cell adhesion and metastasis of bladder cancer. However, the precise mechanism of tumour metastasis regulated by CRT remains unclear. Using a DNA array, we identified FUT1 (fucosyltransferase 1) as a gene regulated by CRT expression levels. CRT regulated cell adhesion through α1,2-linked fucosylation of ?1 integrin and this modification was catalysed by FUT1. To clarify the roles for FUT1 in bladder cancer, we transfected the human FUT1 gene into CRT-RNAi stable cell lines. FUT1 overexpression inCRT-RNAi cells resulted in increased levels of ?1 integrin fucosylation and rescued cell adhesion to type-I collagen. Treatment with UEA-1 (Ulex europaeus agglutinin-1), a lectin that recognizes FUT1-modified glycosylation structures, did not affect cell adhesion. In contrast, a FUT1-specific fucosidase diminished the activation of ?1 integrin. These results indicated that α1,2-fucosylation of ?1 integrin was not involved in integrin-collagen interaction, but promoted ?1 integrin activation. Moreover, we demonstrated that CRT regulated FUT1 mRNA degradation at the 3'-UTR. In conclusion, the results of the present study suggest that CRT stabilized FUT1 mRNA, thereby leading to an increase in fucosylation of ?1 integrin. Furthermore, increased fucosylation levels activate ?1 integrin, rather than directly modifying the integrin-binding sites. ? 2014 Biochemical Society.
SDGs
Other Subjects
alpha levo fucosidase; beta1 integrin; calreticulin; collagen; fucosyltransferase; fucosyltransferase 1; glycosyltransferase; integrin; messenger RNA; RNA; Ulex europaeus agglutinin; Ulex europaeus agglutinin 1; unclassified drug; article; bladder cancer; cancer cell; catalysis; cell adhesion; controlled study; fucosylation; gene expression; human; human cell; human cell culture; in vitro study; molecular recognition; nucleotide sequence; priority journal; protein glycosylation; protein protein interaction; protein structure; RNA degradation; RNA interference; RNA stability; Ulex europaeus; Antigens, CD29; Calreticulin; Cell Adhesion; Cell Line, Tumor; Fucosyltransferases; Humans; Protein Stability; RNA Stability; Urinary Bladder Neoplasms
Publisher
Portland Press Ltd
Type
journal article