Leaf volatile components and inheritance analyses inelargonium grossularioides (L.) L’Hér. ex Ait.
Date Issued
2009
Date
2009
Author(s)
Wu, Wan-Ling
Abstract
Leaf volatile components analysis by headspace solid phase microextraction (SPME) and gas chromatography-mass spectrum (GC-MS) of Pelargonium grossularioides. Furthermore, structure a method for the analysis. To discuss with heritability of cis-3-hexenol, myrcene, 3-hexen-1-ol[acetate], limonene, L-fenchone, cyclohexanone, and δ-cadinene. First, samling 40 discs of 4 mm diametral in 25 mL vial; heating 20 minute at 75℃ after add internal standards and then trap 20 minute by SPME; desorb 20 minute. The samples should analyze immediately after sampling. he germination percentage was 100% nearly if seeding immediately after pick with seed meaturity index J. Seed storage temperature at 18℃ was better then at 5℃. The germination percentage at 18℃ was raised with inceease storage time. The germination rate 80.5% which was process 28 days to storage at 18℃. The highest germination percentage was only 44.5% process 16 weeknesses to storage at 5℃. The germination percentage decease and delay with increase storage times. ALI were analyzed of leaf major volatile components by GC/MS of parents, selfing progeny, reciprocal crosses hybrids, and clone in P. grossularioides. Seven leaf major components trend to normal distribution of selfing progeny and reciprocal crosses hybrids of P. grossularioides. There were related to seven major leaf components, especially in myrcene, limonene, L-fenchone, cyclohexanone, and δ-cadinene. Principal component individual were counted by factor analysis of principal component analysis. The date could be explained to three factors of selfing progeny and reciprocal crosses hybrids. The cumulative were 88.9%及95.4% individual of eigenvalue. Three factors to transform by Varimax, F1 correlates with myrcene, limonene, L-fenchone, cyclohexanone, and δ-cadinene; F2 correlates with cis-3-hexenol; F3 correlates with 3-hexen-1-ol[acetate]. Selection extreme individuals, H and L, base on variable F1. Reciprocal crosses testing with H and L. Two populations, H × H和L × L, correlates with three factors, and they segregate clear. That show selection is effective.he broad heritability is effective to propagate clone of seven major components. The minimum of broad heritability is 11.7% (L-fenchone) and the maximum is 43.2%（δ-cadinene）when analyze a single plant (r=1). The broad heritability could increase triple when analyze 4 clone of the single plant (r=4).
Subjects
Pelargonium grossularioides
SPME
broad heritability
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-98-R95628128-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):6c15dd9cab998fda4f43507d48f9e51c