Antimicrobial Resistance of Pseudomonas aeruginosa of Humans, Canines and Death of Chicken Embryos in Northern Taiwan
Date Issued
2008
Date
2008
Author(s)
Sun, Yu-Mei
Abstract
The aims of the study were to survey and differentiate the phenotype and genotype of Pseudomonas aeruginosa from human, companion and economic animal isolates. A total of ninety four human isolates, twenty nine canines isolates and ninety seven chicken isolates used polymerase chain reaction (PCR) to detect the encoding genes of class A (blaTEM, blaSHV, blaGES, blaVEB, blaPER and blaPSE) and class D (blaOXA-2 like, and blaOXA-10 like) β-lactamases. All isolates were also performed antimicrobial susceptibility testing and pulsed-fielded gel electrophoresis (PFGE). Antimicrobial resistance of human isolates were higher than canine isolates, followed by chicken isolates. blaTEM, blaSHV, blaGES, blaVEB, blaPER ,and blaOXA-2 like were not harboured in any isolates. In human, canine and chicken isolates, blaTEM-1 (90.4%, 69.0% and 71.1%), blaPSE-1 (77.7%, 75.9% and 6.2%) and blaOXA-10 like (98.9%, 86.2% and 18.6%) were detected, respectively. OXA-17 was the most common OXA enzyme in human and canine isolates, followed by OXA-10. However, the results of the prevalence of OXA-10 and OXA-17 in chicken isolates were similar (9.3% and 8.2%). All isolates were grouped into twenty clusters by PFGE. Although some isolates from humans or chickens tended to be gathered in specific clusters, other isolates from three sources were mixed together and the result indicated that genetypes of P. aeruginosa are divergent. TEM-1, PSE-1 and OXA-type β-lactamases were widely distributed in Taiwan. The usage of antimicrobial agents and antimicrobial selective pressure might cause great impact on the results. Further surveillance and monitoring the route of resistant genes transmission between P. aeruginosa from difference species and the correlation between antimicrobials and resistant bacteria will be necessary.
Subjects
P. aeruginosa
antimicrobial susceptibility testing
PFGE
TEM
OXA
canine
avian
Type
thesis
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