Motility and protein phosphorylation in healthy and asthenozoospermic sperm
Journal
Journal of Proteome Research
Journal Volume
8
Journal Issue
11
Pages
5382-5386
Date Issued
2009
Author(s)
Abstract
The majority of male infertility results from poor sperm motility. A direct link between altered protein phosphorylation and aberrant sperm motility has not been established. To address this issue, sperm samples obtained from 20 donors with healthy sperm and 20 donors with aberrantly motile sperm were subjected to computer assisted semen analysis (CASA), proteomic analysis, Western blot, and immunofluorescent staining. Proteomic analysis identified 12 protein spots as having differential phosphorylation, including gamma-tubulin complex associated protein 2 (GCP2). Western blot and immunofluorescence demonstrated differential expression of gamma-tubulin between healthy and aberrantly motile sperm. In conclusion, hypophosphorylated proteins and reduced expression of gamma-tubulin may be associated with low motility sperm. ? 2009 American Chemical Society.
Other Subjects
gamma tubulin complex associated protein 2; protein; unclassified drug; adult; article; asthenospermia; clinical article; computer analysis; controlled study; human; immunofluorescence; male; nucleotide sequence; priority journal; protein phosphorylation; proteomics; semen analysis; spermatozoon motility; Western blotting; Adult; Animals; Asthenozoospermia; Electrophoresis, Gel, Two-Dimensional; Humans; Infertility, Male; Male; Molecular Sequence Data; Phosphorylation; Sperm Motility; Spermatozoa; Tubulin; Young Adult
Type
journal article