Design of a Multiple-Throughput Device for Electrophysiological Screening
Date Issued
2004
Date
2004
Author(s)
Chuang, Hsin-Hung
DOI
en-US
Abstract
The patch clamp technique, in the standard electrophysiological assays, is a well-developed and powerful tool for the study of ion channels and transporters. Despite claims that the patch clamp technique has superior characteristics of high information content, the technique is suffered by the laborious and slow manual manipulations of the glass pipette onto the cell by highly skilled technicians with consequential low throughput.
This thesis presents a multiple acrylic layers device（Recording Chamber） that integrates all the necessary conditions in the patch clamp technique on a Poly（dimethylsiloxane）（PDMS）-based Patch-on-A-Chip（Recording Chip）system. PDMS is an attractive material which to mimic the tip of the glass micropipette that can rapidly forms gigaohm seals to a cell membrane after surface treatment. The recording system is not only to avoid the inherent shortcomings found in the conventional patch clamping but also provides Multiple-throughput apertures for electrophysiological recording to raise the throughput of data points.
We demonstrate a recording chip, which is fabricated by an elastomeric membrane using PDMS material conformal contact onto an apertured glass cover slip after a precise alignment, allows patch clamp recording on Xenopus oocytes expressing inactivation-removed Shaker potassium channels at different apertures of the recording chip asynchronously. Our results have indicated the potential of high-throughput electrophysiological screening with an integration of recording system.
Subjects
多通量電生理篩選
軟微影
膜片箝制
Soft Lithography
Patch Clamp
Multiple-Throughput Electrophysiological Screening
Type
thesis
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