Indium-111-labeled CD166-targeted peptide as a potential nuclear imaging agent for detecting colorectal cancer stem-like cells in a xenograft mouse model
Journal
EJNMMI Research
Journal Volume
10
Journal Issue
1
Date Issued
2020
Author(s)
Abstract
Background: Cancer stem cells (CSCs) are involved in drug resistance, metastasis, and relapse of cancers, which can significantly affect tumor therapy. Hence, to develop specifically therapeutic target probe at CSCs for improvement of survival and quality of life of cancer patients is urgently needed. The CD166 protein has been suggested to be involved in colorectal cancer (CRC) tumorigenesis and to be considered a marker for colorectal CSCs (CRCSCs) detection. In this study, therefore, we attend to apply a nuclear imaging agent probe, Glycine18-Cystine-linked CD166-targeted peptides (CD166tp-G18C), to detect the changes of CD166 level in a CRC xenograft mouse model. Results: We isolated the CD166-positive cells from the HCT15 CRC cell line (CD166+HCT15) and evaluated their morphology and ability of clone formation, migration, protein expression, and drug resistance. The CD166-positive HCT15 cells display the CSCs characteristics. We discovered and designed a CD166-targeted peptide (CD166tp-G18C) as a targeted probe of CRC stem-like cell for cell binding assay. The CD166tp-G18C confirmed the CD166 protein targeting ability in CD166+HCT15 cells. The diethylenetriaminopentaacetic acid (DTPA)-conjugated CD166tp-G18C further was labeled with indium-111 (111In-DTPA-CD166tp-G18C) as nuclear imaging agent for imaging and bio-distribution analysis in vivo. Finally, we observed that the 111In-DTPA-CD166tp-G18C was significantly enhanced in tumor tissues of CD166+HCT15 xenograft mice as compared to the non-CD166tp-G18C control. Conclusions: Our results indicated that the indium-111-labeled CD166tp-G18C may be served as a powerful tool for colorectal CSCs nuclear imaging in the CRC patients. ? 2020, The Author(s).
Subjects
Cancer stem cells; CD166-targeted peptides; Colorectal cancer; Nuclear imaging agent probe
SDGs
Other Subjects
activated leukocyte cell adhesion molecule; indium 111; pentetic acid; animal cell; animal model; Article; binding assay; cancer stem cell; cell migration; cell selection; clonogenesis; colorectal cancer; enzyme linked immunosorbent assay; flow cytometry; fluorescence imaging; HCT 15 cell line; human; human cell; image analysis; immunoblotting; immunofluorescence; male; mouse; nonhuman; phage display; priority journal; protein expression; protein interaction; screening; tumor xenograft
Publisher
Springer
Type
journal article