Hypoxia-Mediated down-Regulation of Octn2 and Ppar Alpha Expression in Human Placentas and in Bewo Cells
Resource
MOLECULAR PHARMACEUTICS v.8 n.1 pp.117-125
Journal
MOLECULAR PHARMACEUTICS
Journal Volume
v.8
Journal Issue
n.1
Pages
117-125
Date Issued
2011
Date
2011
Author(s)
CHANG, TING-TING
SHYU, MING-KWANG
HUANG, MIN-CHUAN
HSU, CHEN-CHI
YEH, SZU-YU
CHEN, MEI-RU
LIN, CHUN-JUNG
Abstract
The objective of the present study was to investigate the effects of hypoxia on placental expression of OCTN2 and PPAR alpha. OCTN2 and PPAR alpha expression in the human placenta in the presence or absence of preeclampsia was examined by immunohistochemical IHC analysis, Western blotting, and quantitative polymerase chain reaction qPCR. Effects of hypoxia on the expression of OCTN2 and PPAR alpha in human placental explants and human choriocarcinoma BeWo cells were examined by Western blotting and qPCR analyses. IHC, Western blot, and qPCR studies showed that OCTN2 and PPAR alpha protein and mRNA levels were lower in syncytiotrophoblasts from preeclamptic human placentas than in those from normal placentas. Hypoxic treatment caused a decrease in OCTN2 and PPAR alpha expression in human placental explants and in BeWo cells. VVY14643, a PPAR alpha agonist, caused an increase in OCTN2 expression in BeWo cells under hypoxic conditions. In conclusion, under hypoxic conditions, placental OCTN2 is down-regulated through PPAR alpha-mediated pathways.
Subjects
OCTN2
PPAR alpha
Preeclampsia
Hypoxia
BeWo cells