Polymerase chain reaction with phase change as intrinsic thermal control
Journal
Applied Physics Letters
Journal Volume
102
Journal Issue
17
Pages
173701
Date Issued
2013
Author(s)
Abstract
This research demonstrated that without any external temperature controller, the capillary convective polymerase chain reaction (ccPCR) powered by a candle can operate with the help of phase change. The candle ccPCR system productively amplified hepatitis B virus 122 base-pairs DNA fragment. The detection sensitivity can achieve at an initial DNA concentration to 5 copies per reaction. The results also show that the candle ccPCR system can operate functionally even the ambient temperature varies from 7 °C to 45 °C. These features imply that the candle ccPCR system can provide robust medical detection services. ? 2013 AIP Publishing LLC.
SDGs
Other Subjects
Viruses; Detection sensitivity; DNA concentration; DNA fragment; External temperature; Hepatitis B virus; Phase Change; Thermal control; Polymerase chain reaction
Publisher
American Institute of Physics Inc.
Type
journal article