毛細液相層析與拋棄式微電灑探針電灑法質譜/質譜於電白質鑑定之開發與應用
Date Issued
2005
Date
2005
Author(s)
蔡竺芸
DOI
zh-TW
Abstract
We set up an on-line micro capillary reversed phase liquid chromatography interfaced to a tandem mass spectrometry (μLC-MS/MS) for protein identification. Proteins from lamellar bodies which were separated by two-dimensional gel electrophoresis were identified by μLC-MS/MS. However, the current technique is rather time consuming. To reduce the analysis time, we developed a disposable device that combined on-line clean up and infusion unseparated proteolytic peptides into an ESI tandem MS. It was demonstrated that several proteins of lamellar bodies could be successfully identified by using this on-line clean up method. Compared to μLC-MS/MS, this method could identify proteins rapidly and without the problem of cross-contamination although the peptide sequence coverage was slightly lower.
Subjects
質譜
蛋白質
mass spectrometry
protein identification
Type
thesis
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