以噬菌體呈現法尋找人類肝白蛋白受體
Other Title
Search for human albumin receptor using phage display system
Date Issued
2002
Date
2002
Author(s)
李嘉哲
DOI
902314B002219MH
Abstract
Human serum albumin in one of major
proteins that are produced by the liver. It
plays an important role in maintaining
intravascular oncotic pressure and acts as a
carrier for various hormones, proteins, fatty
acids, ions and drugs.
We found that expression of albumin in
hepatocyte or hepatocellular carcinoma cell
line correlated inversely with the
concentration of albumin in the culture
medium. This implied that albumin receptor
possibly existed in these cells. Initially, we
used phage display system to find out this
receptor, but failed. We switched to
BacterioMatch two-hybrid system and
revealed two candidate genes, phosphodiesterse 3B and ATP-binding
cassette, member 4. We Have cloned these
genes. We are currently defined their roles as possible receptor of albumin.
Subjects
Albumin
Receptor
BacterioMatch two-hybrid
system
system
Publisher
臺北市：國立臺灣大學醫學院內科
Type
report
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