Genetic Recombination Analysis of Two Recombinant Inbred Lines Populations in Rice
Date Issued
2015
Date
2015
Author(s)
Chen, Hsing-Liang
Abstract
In this study comparative maps of rice were constructed with a reference physical map (Nippobare) using SSR DNA markers and two recombinant inbred lines (RILs) populations, which were generated by a modified Single Seed Descendent (SSD) method. One population including 121 RILs was generated from an inter-subspecific cross between cultivars Taichung Sen No.10 (TCS10) and Koshihikari (KH). While the other population including 146 RILs was generated from an intra-subspecific cross between cultivar Koshihikari and Tai Nung No.67 (TNG67) with the same method.More misposition and conversion events were found in the linkage groups of the population TCS10 × KH. Besides, the genetic contribution of indica variety TCS10 is larger than that of japonica variety KH in the populations TCS10 × KH, and japonica TNG67 also has larger genetic contribution than KH. In addition, TCS10 × KH has more obvious segregation distortion (SD) were observed in the population TCS10 × KH than in the population KH × TNG67. There were several SD events at the corresponding positions on most chromosomes of the two populations, suggesting that SD events were not taken place in random. The rates of recombination event or double crossing over event are higher in the population KH × TNG67. Also, linkage disequilibrium (LD) decay was slower in the population TCS10 × KH than KH × TNG67. According to LD decay data, the amount of SSR molecular markers were sufficient in both populations; these markers were not sufficiently distributed evenly. To sum up, more projenies and selfing generations in a population developed from a cross between indica and japonica varieties. The results of this study could provide informations for rice breeding.
Subjects
recombinant inbred line
rice
genetic recombination
marker-assisted selection
comparative map
linkage disequilibrium
Type
thesis
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